How to use the dna copy number calculator
Convert a DNA mass to number of copies. Enter dna mass, length in the fields above; the molecules, moles are computed instantly and plotted on the chart so you can see how the answer changes.
Common searches this calculator answers include “copies of plasmid”, “qpcr standard”, “molecules of dna”, “calculate dna copy number”, “find molecules”.
Formula
Variables
- m — DNA mass (ng)
- L — Length (bp)
Worked example
With dna mass = 10 ng, length = 5000 bp, the calculator gives molecules ≈ 1.853 × 10⁹; moles ≈ 3.076923 fmol.
Frequently asked questions
What is the dna copy number calculator used for?
Convert a DNA mass to number of copies. It helps with questions such as “copies of plasmid”, “qpcr standard”, “molecules of dna”.
What is the formula for the dna copy number calculation?
Molecules = m × 1e-9 × NAV / (L × 650). Moles (fmol) = [Molecules] / NAV × 1e15. Here m is dna mass (ng), L is length (bp).
What inputs do I need for the dna copy number calculator?
You need dna mass in ng, length in bp. Each field is pre-filled with a typical example so you can see how the result responds as you edit the numbers.
How do I use the dna copy number calculator?
Type values into the input fields. Results and the chart update instantly. Use Reset to restore the example values, Copy results to paste them elsewhere, or Share to copy a link that preserves your inputs.
Is the dna copy number result exact?
Calculations use standard formulas and double-precision arithmetic, then display about seven significant figures. Real-world measurements carry uncertainty, so treat results as estimates unless your inputs are exact.